1 kb Plus DNA Ladder (2024)

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• Product Information

  A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

  Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

  NEB also offers a Quick-Load version of this ladder with purple dye.

  • Recommended gel percentage range: 1-1.4%
  • Optimum separation on 1.2%

  
  

  This product is related to the following categories:

  This product can be used in the following applications:

  DNA Analysis

  • Reagents Supplied

    The following reagents are supplied with this product:

    See Also

    Low Molecular Weight DNA LadderQuick-Load Purple Low Molecular Weight DNA LadderTriDye™ Ultra Low Range DNA Ladder1 kb DNA Ladder | NEB

    NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
    N3200S -20 1 kb Plus DNA Ladder N3200SVIAL -20 1 x 0.1 ml 1 mg/ml Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

  • Properties & Usage

    Bases

    Fragment	Mass (ng)	bp
    1	40	10002
    2	40	8001
    3	48	6001
    4	40	5001
    5	32	4001
    6	120	3001
    7	40	2017
    8	57	1517
    9	45	1200
    10	122	1000
    11	34	900
    12	31	800
    13	27	700
    14	23	600
    15	124	500/517
    16	49	400
    17	37	300
    18	32	200
    19	61	100

    Effective Size Range

    100bp to 10,002bp

    Storage Buffer

    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

  • Related Products

    Companion Products

    • 1 kb DNA Ladder
    • 100 bp DNA Ladder
    • 50 bp DNA Ladder
    • PCR Marker
    • Quick-Load® 1 kb DNA Ladder
    • Quick-Load® 100 bp DNA Ladder
    • Quick-Load®1 kb Plus DNA Ladder
    • TriDye™ 1 kb DNA Ladder
    • TriDye™ 100 bp DNA Ladder
    • TriDye™ 1 kb Plus DNA Ladder
    • Gel Loading Dye, Purple (6X), no SDS
    • Monarch® Plasmid Miniprep Kit
    • Monarch® DNA Gel Extraction Kit

    Materials Sold Separately

    • Gel Loading Dye, Purple (6X), no SDS

  • Product Notes

    1. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    2. We recommend loading 0.5-1 μg of the 1 kb Plus DNA Ladder diluted in sample buffer.
    3. All fragments have 4-base, 5´overhangs that canbe end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA PolymeraseI, Klenow Fragment (#M0210) (1). Use α-[32P] dATP or α-[32P] dTTP for the fill-in reaction.
    4. 1 kb Plus DNA Ladder is stable for at least 3 months at 4°C.
    5. For long term storage, store at -20°C. If samples need to be diluted, useTE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂0.
    6. 1X Gel Loading Dye, Purple (6X), no SDS:
       2.5% Ficoll^®-400
       10mM EDTA
       3.3mM
       Tris-HCL (pH 8.0@25°C)
       0.02% Dye 1
       0.001% Dye 2
    7. Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.

  • References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 10.51-10.67.

• Protocols, Manuals & Usage

  • Protocols

    1. End-labeling Protocol

• Tools & Resources

  • Selection Charts

• FAQs & Troubleshooting

  • FAQs

    1. Why are there extra bands visible on polyacrylamide gels?
    2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    3. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    4. How can I quantify the amount of DNA in each band of a marker?
    5. Can I use Midori Green with the DNA Ladders from NEB?
    6. Can I use SYBR®and/or GelRed®dyes with the DNA Ladders from NEB?
    7. Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
    8. What are the differences in the four versions of 2-Log, 100 bp and 1 kb DNA Ladders that NEB sells?

    See Also

    Low Molecular Weight DNA Ladder

  • Tech Tips

    To make it ready-to-load, dilute in TE buffer instead of water.

• Citations & Technical Literature

  • Citations

    Product Citation Tool

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• Quality, Safety & Legal

  • Quality Control Assays

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

  • Specifications & Change Notifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • N3200S_L_v2

  • Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • N3200S_L_v1_0681401
    • N3200S_L_v1_0691403
    • N3200S_L_v1_0711406
    • N3200S_L_v1_0731409
    • N3200S_L_v1_0741501
    • N3200S_L_v1_0751503
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    • N3200S_L_v1_0791510
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    • N3200S_L_v1_0821604
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    • N3200S_L_v1_0841610
    • N3200S_L_v1_0841611
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    • N3200S_L_v1_0871704
    • N3200S_L_v1_0881704
    • N3200S_L_v1_0891708
    • N3200S_L_v1_0911712
    • N3200S_L_v1_0921803
    • N3200L_v2_10010859
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    • N3200L_v2_10060555
    • N3200S_v2_10062016
    • N3200L_v2_10065657
    • N3200S_v2_10067200
    • N3200L_v2_10073071
    • N3200S_v2_10072654
    • N3200S_v2_10079637
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    • N3200S_v2_10169651
    • N3200L_v2_10175779
    • N3200S_v2_10176594
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    • N3200S_v2_10233849
    • N3200L_v2_10240132
    • N3200S_v2_10241556

  • Safety Data Sheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • 1 kb Plus DNA Ladder

    • Gel Loading Dye, Purple (6X), no SDS

  • Legal and Disclaimers

    Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.