Low Range ssRNA Ladder | NEB (2024)

HomeProductsLow Range ssRNA Ladder

• Product Information

  The Low Range ssRNA Ladder is aset of 6 RNA molecules produced by in vitro transcriptionof a mixture of 6 linear DNA templates.The ladder sizes are: 1000, 500, 300, 150, 80 and50 bases. The 300 base fragment is at doubleintensity to serve as a reference band. This ladderis suitable for use as an ssRNA size standardon denaturing polyacrylamide-urea gels, and ondenaturing or native agarose gels.

  Usage Recommendation
  This marker was notdesigned for precise quantification of ssRNAmass.

  Denaturing vs. Native Agarose Gels
  It is commonpractice to electrophorese RNA on a fullydenaturing agarose gel, such as one containingformaldehyde (1). However, in many cases itis possible to run RNA on a native agarose geland obtain suitable results. In fact, it has beendemonstrated that treatment of RNA samples ina denaturing buffer maintains the RNA moleculesin a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarosegels eliminates problems associated with toxicchemicals and the difficulties encountered whenstaining and blotting formaldehyde gels.

  Sample Preparation
  The Low Range ssRNALadder is also compatible with formaldehydebasedloading buffers.

  
  

  This product is related to the following categories:

  RNA Markers and Ladders

  • Reagents Supplied

    The following reagents are supplied with this product:

    See Also

    Low Molecular Weight DNA LadderQuick-Load Purple Low Molecular Weight DNA LadderTriDye™ Ultra Low Range DNA Ladder1 kb DNA Ladder | NEB

    NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
    N0364S -20 Low Range ssRNA Ladder N0364SVIAL -20 1 x 0.05 ml Not Applicable RNA Loading Dye, (2X) B0363AVIAL -20 1 x 1 ml 2 X

  • Properties & Usage

    Storage Buffer

    20 mM sodium citrate
    1 mM EDTA
    pH 6 @ 25°C

  • Related Products

    Companion Products

    • microRNA Marker
    • RNA Loading Dye, (2X)

    Materials Sold Separately

    • RNA Loading Dye, (2X)

  • Product Notes

    1. Minimize repeated freeze-thawcycles. It is best to aliquot the marker into singleuse portions.

       To avoid ribonuclease contamination: wear gloves,use RNase-free water for gels and buffers, washequipment with detergent and rinse thoroughlywith RNase-free water.

    2. Store at -20˚C for long-term storage. For short-term storage (<1 week), store at 4˚C.

  • References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd ed., 743-745.
    2. Liu, Y-C. and Chou, Y-C. (1990). Biotechniques. 9,
    3. Sandra Cook, and Christina Marchetti Unpublished observation
    4. Dong Ma, and John Greci Unpublished observation

• Protocols, Manuals & Usage

  • Protocols

    1. Sample preparation using ssRNA Ladder provided buffer (NEB #N0364)

• FAQs & Troubleshooting

  • FAQs

    1. What types of gels are recommended for ssRNA and dsRNA ladders? (N0363, N0362, N0364)
    2. What types of gel and staining dyes can I use for NEB’s Low Range ssRNA Ladder (N0364S)?
    3. Why the bands of the Low Range ssRNA Ladder on my 2% agarose-TBE gel are not resolved well?
    4. Why do I have extra bands of the Low Range ssRNA Ladder on my denaturing 6% TBE-Urea gel?
    5. Will the Low Range ssRNA Ladder run on 10% TBE-Urea and 15% TBE-Urea gels?
    6. What is the concentration of RNA in the ssRNA Ladder (NEB #N0362) and Low Range ssRNA Ladder (NEB #N0364)?

• See Also

  Low Molecular Weight DNA Ladder

  Citations & Technical Literature

  • Citations

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• Quality, Safety & Legal

  • Quality Control Assays

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

  • Specifications & Change Notifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • N0364S_v2

  • Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • N0364S_v1_0271601
    • N0364S_v1_0271605
    • N0364S_v1_0281609
    • N0364S_v1_0281704
    • N0364S_v1_0281709
    • N0364S_v1_0281802
    • N0364S_v1_10013508
    • N0364S_v1_10018990
    • N0364S_v1_10024418
    • N0364S_v1_10031678
    • N0364S_v1_10041516
    • N0364S_v2_10052279
    • N0364S_v2_10056647
    • N0364S_v2_10062652
    • N0364S_v2_10069539
    • N0364S_v2_10077426
    • N0364S_v2_10080102
    • N0364S_v2_10082477
    • N0364S_v2_10095257
    • N0364S_v2_10105450
    • N0364S_v2_10116075
    • N0364S_v2_10134342
    • N0364S_v2_10140334
    • N0364S_v2_10149110
    • N0364S_v2_10162765
    • N0364S_v2_10175912
    • N0364S_v2_10188376
    • N0364S_v2_10190211
    • N0364S_v2_10205286
    • N0364S_v2_10212161
    • N0364S_v2_10224633
    • N0364S_v2_10225994
    • N0364S_v2_10239126
    • N0364S_v2_10241681

  • Safety Data Sheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Low Range ssRNA Ladder

    • RNA Loading Dye, (2X)

  • Legal and Disclaimers

    Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.