Quick-Load Purple Low Molecular Weight DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.
The DNA Ladder consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 25-766 base pairs. The 200 base pair band has increased intensity to serve as a reference point..
- Recommended gel percentage range: 2-3.5%
- Optimum separation on 3%
Reagents Supplied
The following reagents are supplied with this product:
| NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
| N0557S | 4 |
| Quick-Load® Purple Low Molecular Weight DNA Ladder | N0557SVIAL | 4 | 1 x 1.25 ml | 50 µg/ml | |
| Gel Loading Dye, Purple (6X), no SDS | B7025SVIAL | 25 | 1 x 1 ml | 6 X |
Properties & Usage
Bases
| Fragment | Mass (ng) | bp |
|---|---|---|
| 1 | 42 | 766 |
| 2 | 27 | 500 |
| 3 | 20 | 350 |
| 4 | 33 | 300 |
| 5 | 27 | 250 |
| 6 | 110 | 200 |
| 7 | 33 | 150 |
| 8 | 43 | 100 |
| 9 | 58 | 75 |
| 10 | 63 | 50 |
| 11 | 43 | 25 |
Effective Size Range
25bp to 766bp
Storage Buffer
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C
Companion Products
- Low Molecular Weight DNA Ladder
- Quick-Load® Purple 100 bp DNA Ladder
- Gel Loading Dye, Purple (6X), no SDS
- Quick-Load Purple 50 bp DNA Ladder
Notes
- Preparation of DNA: The double-stranded DNA is digested to completionwith appropriate restriction enzymes, phenol extracted and equilibratedin storage buffer.
- We recommend loading 10 μl (0.5 μg) of Quick-Load Purple Low Molecular Weight DNALadder per gel lane. In general, it is recommended to load 1 µl per mmof gel lane.
- The Quick-Load Purple Low Molecular Weight DNA Ladder is not intended for precisequantification of DNA mass but can be used for approximating the mass ofDNA in comparably intense samples of similar size.
- Quick-Load Purple Low Molecular Weight DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
- 1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2
References
- Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), pp.10.51-10.67, Cold Spring Harbor Laboratory Press.
Protocols
- Protocol for Quick-Load Purple Low Molecular Weight DNA Ladder (NEB #N0557)
Selection Charts
FAQs
- Why are there extra bands visible on polyacrylamide gels?
- Can I use Midori Green with the DNA Ladders from NEB?
- Can I use SYBR®and/or GelRed®dyes with the DNA Ladders from NEB?
- Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
- Can I use GelRed®with the Low Molecular Weight and 50 bp DNA Ladders from NEB?
Citations
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- N0557S_v1_0011608
- N0557S_v1_0011612
- N0557S_v1_0011704
- N0557S_v1_0011709
- N0557S_v1_0011803
- N0557S_v1_0021804
- N0557S_v1_10019441
- N0557S_v1_10045826
- N0557S_v1_10062737
- N0557S_v1_10085990
- N0557S_v1_10100712
- N0557S_v1_10104050
- N0557S_v1_10126710
- N0557S_v1_10152597
- N0557S_v1_10176564
- N0557S_v1_10212058
- N0557S_v1_10241003
Legal And Disclaimer
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
